The 28-year-old Australian announced on Saturday that he will miss the tournament, which gets under way on January 14.
Kyrgios pulled out of the Melbourne major at the start of this year and was out for five months after undergoing surgery on his left knee.
He then missed the French Open due to a foot injury and pulled out of Wimbledon with a wrist ligament problem that also forced him out of the US Open.
“This is a very disappointing time for me, but I won’t be able to compete at the 2024 Australian Open,” Kyrgios said on the online platform OnlyFans, which he joined on Friday.